Stoneflies as model organisms for studying the evolution of insect flight

How flight evolved in insects is of particular interest for two reasons. Firstly, acquisition of flight triggered a relatively rapid speciation and radiation that resulted in insects becoming the most speciose life form on the planet (approximately two-thirds of all described species). Secondly, flight is a foremost example of one of the primary challenges for Darwinian evolution, which is to determine transitions in function and selective advantage for intermediate stages during evolution of complex suites of interdependent anatomical, physiological, and behavioral features (i.e. how do complex mechanisms evolve in a stepwise fashion?). The fossil record and a variety of other evidence indicates that insect wings most likely evolved from movable gill plates that aquatic forms used for ventillation and/or swimming. We have recently discovered that a group of morphologically primitive aquatic insects (stoneflies) uses a form of locomotion that is intermediate between swimming and flying. These stoneflies cannot fly, but instead use their wings as sails (non-flapping variations in wing posture) or as propellers (flapping) to skim across water surfaces in a planar fashion. For a wing-flapping species, we have shown that surface-skimming performance improves steadily with incremental increases in wing size, muscle size, and muscle power output. Surface-skimming is possible even when wing area is reduced by as much as 80%, and when muscle power output is minimal. Thus, we have concluded that this form of locomotion would have allowed directional selection, beginning with forms possessing only gills, towards fully flying forms. Our present goal is to determine whether surface skimming locomotion represents retention of a primitive state (the "missing link hypothesis") or is an evolutionary digression from flying ancestors (the "penguin hypothesis"). To distinguish between these two alternatives, we are constructing a molecular phylogeny for stonefly families/subfamilies using 18S DNA sequence data, onto which we will map data regarding 1) how the various taxa locomote, and 2) functionally relevant aspects of wing design.




Publications resulting from this project to date:

Marden, J.H. and M.G. Kramer. 1994. Surface-skimming stoneflies: a possible intermediate stage in insect flight evolution. Science 266, 427-430. (abstract)

Marden, J.H. 1995. Flying lessons from a flightless insect. Natural History 104 (2), 4-8

Marden, J.H. and M.G. Kramer. 1995. Locomotor performance of insects with rudimentary wings: sailing on water versus gliding in air. Nature 377, 332-334. (abstract & cover)

Marden, J.H. How insects learned to fly. The Sciences 35, 26-30.

Marden, J.H. and M.G. Kramer. 1995. Plecopteran surface-skimming and insect flight evolution - reply. Science 270, 1685.

Kramer, M.G. and J.H. Marden. 1996. Almost airborne. Nature, 385, 403-404. Acknowledgements

Quick-time movies of Surface-skimming Stoneflies

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